Regulatory

Part:BBa_K319011

Designed by: Matthew Orton   Group: iGEM10_uOttawa   (2010-10-20)


yeast T7 Proximal Promoter

The proximal T7 promoter was constructed with two tandem TetR operators in addition to the TATA box and start site of the GAL1 promoter. The yeast promoter is repressed by TetR (Tn10.B tetracycline repressor) and inducible with the TetR-inhibitor andhydrotetracylcine (ATc). The promoter was constructed with runs of unspecified ("N") sequence separating motifs and fixed tandem TetR operator and GAL1 motifs. The unspecified motifs modulate the efficiency of the fixed motifs to fine tune the expression of the downstream protein coding region.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None